Gvt-journal.biomedcentral.com

HCV entry receptors as potential targets for siRNA …

WebHCV infection is a major health problem; more than 350 million people worldwide and 10% of the population in Pakistan are chronically infected with this disease [1, 2].In 40-60% of …

Actived: 9 days ago

URL: https://gvt-journal.biomedcentral.com/articles/10.1186/1479-0556-9-15

The significance of controlled conditions in lentiviral vector

WebThe inoculum volume of the vector and the number of target cells affect vector titre determination, but not proportionally. Figure 1 shows that during the lentiviral vector …

Category: Health Go Health

Targeting wild-type Erythrocyte receptors for Plasmodium

WebBackground Malaria causes immense human morbidity and mortality globally. The plasmodium species vivax and falciparum cause over 75 % clinical malaria cases. Until …

Category: Health Go Health

Is gene therapy a good therapeutic approach for HIV-positive …

WebDespite advances and options available in gene therapy for HIV-1 infection, its application in the clinical setting has been challenging. Although published data from HIV-1 clinical …

Category: Health Go Health

A brief review on dengue molecular virology, diagnosis, treatment …

WebDengue virus infection is a serious health problem infecting 2.5 billion people worldwide. Dengue is now endemic in more than 100 countries, including Pakistan. Each year …

Category: Health Go Health

Genetic Vaccines and Therapy BioMed Central

WebBioMed Central Page 1 of 13 (page number not for citation purposes) Genetic Vaccines and Therapy Research Open Access DNA vaccine constructs against enterovirus 71 elicit …

Category: Health Go Health

Establishment of stable Huh-7 cell lines expressing various …

WebHepatitis C virus (HCV) is an enveloped plus-strand RNA virus of family Flaviviridae [1, 2].HCV is a major leading cause of chronic liver disease [].An estimated 170-200 million …

Category: Health Go Health

Characterization of a potent non-cytotoxic shRNA directed to the …

WebCCR5 shRNA expressed from U6 promoter are effective but cytotoxic to primary PBMCs. Using published algorithms[] to predict potent shRNA sequences, we tested 8 shRNAs …

Category: Health Go Health

In-vitro model systems to study Hepatitis C Virus

WebHepatitis C virus (HCV) is a major cause of chronic liver diseases including steatosis, cirrhosis and hepatocellular carcinoma. Currently, there is no vaccine available for …

Category: Health Go Health

Evaluation of the immune responses induced by four targeted …

WebLiver fluke can infect cattle and sheep, and is also emerging as a human pathogen in developing countries. Cathepsin B (Cat B2) is a major cysteine protease secreted by the …

Category: Health Go Health

Electroporation by nucleofector is the best nonviral transfection

WebIn CoASMC, use of FuGENE 6 achieved the best results. It produced almost twice as much CAT per ml media than cells transfected using the second best performing reagent, …

Category: Health Go Health

The recombinant adeno-associated virus vector (rAAV2)-mediated

WebBackground In humans, overproduction of apolipoprotein B (apoB) is positively associated with premature coronary artery diseases. To reduce the levels of apoB mRNA, we have …

Category: Health Go Health

DNA vaccine constructs against enterovirus 71 elicit

WebEnterovirus 71 (EV71) is a major causative viral agent responsible for large outbreaks of hand, foot and mouth disease (HFMD), a common rash illness in children and infants. …

Category: Health Go Health

Genetic immunization with Hantavirus vaccine combining …

WebIn this research, we developed a novel chimeric HTNV-IL-2-G2 DNA vaccine plasmid by genetically linking IL-2 gene to the G2 segment DNA and tested whether it could be a …

Category: Health Go Health

Molecular relationship between field and vaccine strain of measles

WebCountrywide 5.9 million, 0-11 Month old children are immunized annually by EPI (Expended Program on Immunization) against 8 vaccine preventable diseases including measles …

Category: Health Go Health

In vitro evaluation of a double-stranded self-complementary …

WebBackground Both adenoviral and lentiviral vectors have been successfully used to induce bone repair by over-expression of human bone morphogenetic protein 2 (BMP-2) in …

Category: Health Go Health

Recombinant adeno-associated virus type 2-mediated gene …

WebBackground Leber's congenital amaurosis (LCA) is a severe form of retinal dystrophy. Mutations in the RPE65 gene, which is abundantly expressed in retinal pigment epithelial …

Category: Health Go Health

Comparison of bovine leukemia virus (BLV) and CMV promoter …

WebViral promoters are used in mammalian expression vectors because they generally have strong activity in a wide variety of cells of differing tissues and species. The utility of the …

Category: Health Go Health

Lentiviral-mediated gene correction of mucopolysaccharidosis …

WebMucopolysaccharidosis type IIIA (MPS IIIA) is the most common of the mucopolysaccharidoses. The disease is caused by a deficiency of the lysosomal enzyme …

Category: Health Go Health

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